User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/29: Difference between revisions

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* 5-16 - Cas-tone
* 5-16 - Cas-tone


# DBN007_1 - VF2
# DBN007-1 - VF2
# DBN007_1 - VR
# DBN007-1 - VR
# DBN007_2 - VF2
# DBN007-2 - VF2
# DBN007_2 - VR
# DBN007-2 - VR
# H3140_1 - CMV_f
# H3140-1 - CMVf
# H3140_1 - DD123_r
# H3140-1 - DD123
# H3140_2 - CMV_f
# H3140-2 - CMVf
# H3140_2 - DD123_r
# H3140-2 - DD123
# H3160_1 - CMV_f
# H3160-1 - CMVf
# H3160_1 - DD123_r
# H3160-1 - DD123
# H3160_2 - CMV_f
# H3160-2 - CMVf
# H3160_2 - DD123_r
# H3160-2 - DD123_r
# H3180_1 - CMV_f
# H3180-1 - CMV_f
# H3180_1 - DD123_r
# H3180-1 - DD123_r
# H3180_2 - CMV_f
# H3180-2 - CMV_f
# H3180_2 - DD123_r
# H3180-2 - DD123_r
# H31116_2 - CMV_f
# H31116-2 - CMV_f
# H31116_2 - DD123_r
# H31116-2 - DD123_r
# H31136_1 - CMV_f
# H31136-1 - CMV_f
# H31136_1 - DD123_r
# H31136-1 - DD123_r




Revision as of 15:14, 29 May 2015

Karmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

mm/dd/yy

  • David Tze - minpreps & digests
  • Ben - sequencing order
  • Cas-tone - sequencing order


David Tze - minpreps & digests

  • Minipreps
    • Sigma GenElute kit, elute w/ 75 μL elution sln.
  • Digests
    • Check with E/S digests; cannot use PstI because PLrigid & PLrigid4 contain PstI sites
Reagent Volume Expected:
1. DT001 = size
2. DT002 = size
3. DT003 = size
4. DT004 = size
Empty vectors = ~2600, ~400 - 600 		Hover name
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
SpeI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.

Sequencing order

  • 1-4 - Ben
  • 5-16 - Cas-tone
  1. DBN007-1 - VF2
  2. DBN007-1 - VR
  3. DBN007-2 - VF2
  4. DBN007-2 - VR
  5. H3140-1 - CMVf
  6. H3140-1 - DD123
  7. H3140-2 - CMVf
  8. H3140-2 - DD123
  9. H3160-1 - CMVf
  10. H3160-1 - DD123
  11. H3160-2 - CMVf
  12. H3160-2 - DD123_r
  13. H3180-1 - CMV_f
  14. H3180-1 - DD123_r
  15. H3180-2 - CMV_f
  16. H3180-2 - DD123_r
  17. H31116-2 - CMV_f
  18. H31116-2 - DD123_r
  19. H31136-1 - CMV_f
  20. H31136-1 - DD123_r





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