User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/28: Difference between revisions
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* DBN001_psB1A3: https://benchling.com/s/S2BS6ziD/edit | * DBN001_psB1A3: https://benchling.com/s/S2BS6ziD/edit | ||
* DBN006: https://benchling.com/s/XoQEXLOz/edit | * DBN006: https://benchling.com/s/XoQEXLOz/edit | ||
* Minipreps<br> | |||
** Sigma GenElute kit, elute w/ 75 μL elution sln. | |||
* Digests | * Digests | ||
** Cut w/ E/S | ** Cut w/ E/S | ||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | <u>Expected:</u><br>1-2. DBN007_pSB1A3 = 2197, 2132<br>DBN001 = 2132, 224<br> | |||
| rowspan="7" | [[Image:somegel.jpg|250px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
|- | |||
| DNA(plasmid) || 2.0 μL | |||
|- | |||
| 10X buffer || 1.5 | |||
|- | |||
| EcoRI || 1.0 | |||
|- | |||
| SpeI || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 9.5 | |||
|- | |||
| || 15 μL | |||
|} | |||
--> 37°C/ ~15 min. | |||
Revision as of 20:18, 28 May 2015
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05/28/15
David Tze - assemblies: linkers + hPCD
--> 37°C/ ~30 min.
--> 37°C/ ~15 min.
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