User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/27: Difference between revisions
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RESULTS: | RESULTS: | ||
# Lu34_AA01 - 12 -- Melt temps 85.1 ± 0.1 °C | # Lu34_AA01 - 12 -- Melt temps 85.1 ± 0.1 °C; additional short peak ~87°C | ||
# Ga34_AA01 - 12 -- Melt temps 85.1 ± 0.1 °C | # Ga34_AA01 - 12 -- Melt temps 85.1 ± 0.1 °C; additional short peak ~87°C | ||
# nTc (3 wells) -- no amplification | # nTc (3 wells) -- no amplification | ||
* Continue by sequencing a few PCR products with P215 to find a reference sample (wild type sequence) | * Try using more diluted template (100x) | ||
* Continue by sequencing a few purified PCR products with P215 to find a reference sample (wild type sequence) | |||
Revision as of 15:25, 28 May 2015
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05/27/15
Rene - hi-res melt curve screen on CRISPR clones
RESULTS:
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