User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Master Mix: Primers+SYBR | * Master Mix: Primers+SYBR | ||
** F = P149 | |||
** R = P160 | |||
** 10.5 per well | ** 10.5 per well | ||
{| | {| {{table}} | ||
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| Reagent || Volume || (x30) | | Reagent || Volume || (x30) | ||
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RESULTS: | RESULTS: | ||
# Lu34_AA01 - 12 -- Melt temps 85.1 ± 0.1 °C | # Lu34_AA01 - 12 -- Melt temps 85.1 ± 0.1 °C; additional short peak ~87°C | ||
# Ga34_AA01 - 12 -- Melt temps 85.1 ± 0.1 °C | # Ga34_AA01 - 12 -- Melt temps 85.1 ± 0.1 °C; additional short peak ~87°C | ||
# nTc (3 wells) -- no amplification | # nTc (3 wells) -- no amplification | ||
* Continue by sequencing a few PCR products to find a reference sample (wild type sequence) | * Try using more diluted template (100x) | ||
* Continue by sequencing a few purified PCR products with P215 to find a reference sample (wild type sequence) | |||
Latest revision as of 00:59, 27 September 2017
Karmella's BioBrick Cloning | Main project page Previous entry Next entry | |||||||||||
05/27/15
Rene - hi-res melt curve screen on CRISPR clones
RESULTS:
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