User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/26: Difference between revisions
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| bgcolor=#cfcfcf | Rxn1-24 | | bgcolor=#cfcfcf | Rxn1-24 | ||
| bgcolor=#cfcfcf | Mix (x25) | | bgcolor=#cfcfcf | Mix (x25) | ||
| rowspan="7" | Expected:<br>1 | | rowspan="7" | Expected:<br>1-14. PCR insert/ pJET = ~600 bp | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:KAH052615_gel3.jpg|250px|Hover name]]<br>10 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| Template (culture) || 0.5 || --- | | Template (culture) || 0.5 || --- | ||
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* 72°C, 3 min | * 72°C, 3 min | ||
* 4°C ∞ | * 4°C ∞ | ||
CONCLUSIONS | |||
* Success with dirty PCR | |||
* Continue with remainder of dirty PCR & possible SYBR PCR tomorrow | |||
Revision as of 17:49, 26 May 2015
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05/26/15
Ben - luc replacement donor plasmid
Bio-Rad: "Phusion" (block #)
CONCLUSION
Rene - CRISPR PCR library PCR trial
1-12. Luc14 g034 - Lu34_AA01 - Lu34_AA12
Labnet: "GoTaq"
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