User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/22: Difference between revisions
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'''Minipreps'''<br> | '''Minipreps'''<br> | ||
* Check with EcoRI digests - promoter insertion generates a new EcoRI site | * Check with EcoRI digests - promoter insertion generates a new EcoRI site | ||
* Note: BraR = 776, pBra = | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1-8. pBra-BraR_MRV = | | rowspan="7" | <u>Expected:</u><br>1-8. pBra-BraR_MRV = 2989, 988+pro<br>empty vector = 3977<br> | ||
| rowspan="7" | <!-- [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] --> | | rowspan="7" | <!-- [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] --> | ||
|- | |- |
Revision as of 10:58, 22 May 2015
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05/22/15
Minipreps
Assemblies
Oligo annealing
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