User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/22: Difference between revisions
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(Autocreate 2015/05/22 Entry for User:Karmella_Haynes/Notebook/BioBrick_cloning) |
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== | ==05/22/15== | ||
<!-- Precede finished items with a checkmark ✓ --> | <!-- Precede finished items with a checkmark ✓ --> | ||
* | * Ryan - minipreps & digests | ||
* | * Cas-tone - minipreps & digests | ||
* Ben - luc replacement donor plasmid | |||
---- | ---- | ||
'''Minipreps'''<br> | '''Minipreps'''<br> | ||
* Check with | * Check with EcoRI digests - promoter insertion generates a new EcoRI site | ||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. | | rowspan="7" | <u>Expected:</u><br>1-8. pBra-BraR_MRV = 2213+Reg, ~1100<br>empty vector = 3201+Reg<br> | ||
| rowspan="7" | <!-- [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] --> | | rowspan="7" | <!-- [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] --> | ||
|- | |- | ||
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| EcoRI || 1.0 | | EcoRI || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || 10.5 | |||
| dH<sub>2</sub>O || | |||
|- | |- | ||
| || 15 μL --> 37°C/ ~15 min. | | || 15 μL --> 37°C/ ~15 min. | ||
|} | |} | ||
---- | ---- |
Revision as of 10:49, 22 May 2015
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05/22/15
Minipreps
Assemblies
Oligo annealing
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