User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/21: Difference between revisions
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|- bgcolor=#cfcfcf | |- bgcolor=#cfcfcf | ||
| Sample || OD260 || 260/280 || ng/μL | | Sample || OD260 || 260/280 || ng/μL | ||
|- | |||
| 1. MV11 (S/N) || 0.03 || 1.879 || 13.5 | |||
|- | |- | ||
| 2. H3-1-40 PCR || 0.03 || 1.879 || 29.8 | | 2. H3-1-40 PCR || 0.03 || 1.879 || 29.8 | ||
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| 4. H3-1-80 PCR || 0.047 || 1.865 || 46.8 | | 4. H3-1-80 PCR || 0.047 || 1.865 || 46.8 | ||
|- | |- | ||
| | | 5. H3-1-116 PCR || 0.06 || 1.887 || 59.57 | ||
|- | |- | ||
| | | 6. H3-1-136 PCR || 0.062 || 1.932 || 62.0 | ||
|} | |} | ||
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* Ligations | * Ligations | ||
** > 2:1 ration calculations... | ** > 2:1 ration calculations... | ||
** 433 bp largest insert / 9506 bp vector * 2 * | ** 433 bp largest insert / 9506 bp vector * 2 * 50 ng vector = 4.5 ng | ||
** 129 bp smallest insert / 9506 bp vector * 2 * | ** 129 bp smallest insert / 9506 bp vector * 2 * 50 ng vector = 1.4 ng | ||
{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
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| Insert DNA || 0.5 || --- | | Insert DNA || 0.5 || --- | ||
|- | |- | ||
| Vector DNA || | | Vector DNA || 3.5 || 3.5 | ||
|- | |- | ||
| 2x lgn buf (Roche) || | | 2x lgn buf (Roche) || 5.0 || 5.0 | ||
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 | | T4 ligase (NEB) || 1.0 || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || --- || 0.5 | ||
|- | |- | ||
| || | | || 10.0 μL || 10.0 μL | ||
|} | |} | ||
Revision as of 17:52, 21 May 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/21/15
Cas-tone Project
Thermal cycler: LabNet OptiMax "AnOlig shrt"
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