User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/21: Difference between revisions
From OpenWetWare
Line 69: | Line 69: | ||
| Sample || OD260 || 260/280 || ng/μL | | Sample || OD260 || 260/280 || ng/μL | ||
|- | |- | ||
| | | 2. H3-1-40 PCR || 0.03 || 1.879 || 29.8 | ||
|- | |- | ||
| | | 3. H3-1-60 PCR || 0.04 || 1.974 || 39.7 | ||
|- | |- | ||
| | | 4. H3-1-80 PCR || 0.047 || 1.865 || 46.8 | ||
|- | |||
| 4. H3-1-## PCR || 0.06 || 1.887 || 59.57 | |||
|- | |||
| 4. H3-1-## PCR || 0.062 || 1.932 || 62.0 | |||
|} | |} | ||
Revision as of 16:15, 21 May 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/21/15
Cas-tone Project
Thermal cycler program: Note: In the Haynes lab, use the LabNet OptiMax Thermocycler, Program "AnOlig RD"
Your insert is now ready to use. The final concentration is 25 ng/μL.
Oligo annealing
|