User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/21: Difference between revisions
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** Add 1 μL DpnI to each PCR rxn. Incubate @ 37°C/ 5 min. | ** Add 1 μL DpnI to each PCR rxn. Incubate @ 37°C/ 5 min. | ||
** Use the Qiagen Qiaquick PCR Purification kit (cat. no. 28104 ) to purify DNA. | ** Use the Qiagen Qiaquick PCR Purification kit (cat. no. 28104 ) to purify DNA. | ||
** Elute with 30 μL of elution solution. | ** Elute & back-elute with 30 μL of elution solution. | ||
Revision as of 16:07, 21 May 2015
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05/21/15
Cas-tone Project
Thermal cycler program: Note: In the Haynes lab, use the LabNet OptiMax Thermocycler, Program "AnOlig RD"
Your insert is now ready to use. The final concentration is 25 ng/μL.
Oligo annealing
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