User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/21: Difference between revisions
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| rowspan="7" | <!-- [[Image:GelImage.jpg|270px|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] --> | | rowspan="7" | <!-- [[Image:GelImage.jpg|270px|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] --> | ||
|- | |- | ||
| DNA (plasmid) || | | DNA (plasmid) || 15.0 | ||
|- | |- | ||
| 10x buffer || 3.0 | | 10x buffer || 3.0 | ||
|- | |- | ||
| | | SpeI || 1.0 | ||
|- | |- | ||
| | | NotI || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 10.0 | ||
|- | |- | ||
| || 30 μL --> 37°C/ ~ | | || 30 μL --> 37°C/ ~15 min. | ||
|} | |} | ||
Revision as of 15:23, 21 May 2015
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05/21/15
Cas-tone Project
Thermal cycler program: Note: In the Haynes lab, use the LabNet OptiMax Thermocycler, Program "AnOlig RD"
Your insert is now ready to use. The final concentration is 25 ng/μL.
Oligo annealing
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