User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/19: Difference between revisions
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'''Rene - PCR libraries''' | '''Rene - PCR libraries''' | ||
* Inoculate 200 μL mini-cultures | * Inoculate 200 μL mini-cultures with individual colonies picked from PCR cloning plates with sterile micropipette tips | ||
# Luc14g034: 192 colonies, 2 96-well plates (A and B) | # Luc14g034: 192 colonies, 2 96-well plates (A and B) | ||
# Gal4EEDg034: 192 colonies, 2 96-well plates (A and B) | # Gal4EEDg034: 192 colonies, 2 96-well plates (A and B) | ||
* Seal with parafilm, grow at 37°C with shaking | |||
* Spot cultures from 96-well plates onto LB agar amp (100μg/mL) using multichannel pipettor | * Spot cultures from 96-well plates onto LB agar amp (100μg/mL) using multichannel pipettor | ||
** Transferred 2 μL from each well to a 6x8 "array" | ** Transferred 2 μL from each well to a 6x8 "array" |
Revision as of 14:56, 21 May 2015
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05/19/15
Cas-tone - cultures, minipreps, digests
--> 37°C/ ~10 min.
Rene - PCR libraries
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