User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/15: Difference between revisions
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'''Minipreps'''<br> | '''Minipreps'''<br> | ||
* Sigma GenElute mini kit; elute w/ 75μL elution sln. | * Sigma GenElute mini kit; elute w/ 75μL elution sln. | ||
* Check with E/S digests - SpeI site is destroyed in successful ligation | * Check with E/S digests - SpeI site (@250) is destroyed and new SpeI site (@844) is gained in successful ligation | ||
# CMV-dCas9-VP64 1-1 | # CMV-dCas9-VP64 1-1 | ||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1-4. CMV-dCas9-VP64 = | | rowspan="7" | <u>Expected:</u><br>1-4. CMV-dCas9-VP64 = ~5500, 3016, 1363<br>dCas9-VP64 = 4697, 3016, 2099 | ||
| rowspan="7" | [[Image:KAH051515_gel2.jpg|250px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:KAH051515_gel2.jpg|250px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- |
Revision as of 15:53, 18 May 2015
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05/15/15
Assemblies
--> 37°C/ ~30 min.
Minipreps
--> 37°C/ ~10 min.
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