User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/12: Difference between revisions
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* Cas-tone - streak Addgene plasmids for single colonies; design histone primers | * Cas-tone - streak Addgene plasmids for single colonies; design histone primers | ||
* Ryan - re-treat insert DNA (heat, slow anneal); redo dig/lig | * Ryan - re-treat insert DNA (heat, slow anneal); redo dig/lig | ||
* Minipreps - KAH87/MV10 from 2x 5 mL cultures (CMV-PcTF for Ben) | |||
Revision as of 15:15, 12 May 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/12/15
Ryan - retry dig/lig
--> Pipette 5.5 μL master mix into each PCR tube LabNet OptiMax Thermocycler: BbsI Dig/Lig
RESULTS (5/13/15)
Minipreps
Assemblies
Oligo annealing
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