User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/11: Difference between revisions
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| bgcolor=#cfcfcf | Mix (x2) | | bgcolor=#cfcfcf | Mix (x2) | ||
| rowspan=7 | Expected:<br>1. CMV = 588 | | rowspan=7 | Expected:<br>1. CMV = 588 | ||
| rowspan=7 | [[Image: | | rowspan=7 | [[Image:KAH051115_gel1.jpg|150px|Hover name]]<br>5 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| plasmid DNA || 0.5 || 1.0 | | plasmid DNA || 0.5 || 1.0 | ||
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| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1,2. pAub/AubR/MRV = 4240<br>3,4. pBja/BjaR/MRV = 4099<br>5,6. pBra/BraR/MRV = 4191<br>7,8. pRpa/RpaR/MRV = 4116<br>9. Empty MRV = 2230, 971<br><br>np pro. Reg/MRV = ~4000, 971<br> | | rowspan="7" | <u>Expected:</u><br>1,2. pAub/AubR/MRV = 4240<br>3,4. pBja/BjaR/MRV = 4099<br>5,6. pBra/BraR/MRV = 4191<br>7,8. pRpa/RpaR/MRV = 4116<br>9. Empty MRV = 2230, 971<br><br>np pro. Reg/MRV = ~4000, 971<br> | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:KAH051115_gel2.jpg|250px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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| DNA(plasmid) || 2.0 μL | | DNA(plasmid) || 2.0 μL | ||
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Conclusions | Conclusions | ||
* Clones 1, 7, 8 look like empty Regulator-MRV vectors | |||
Revision as of 16:28, 12 May 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/11/15
Cas-tone Project
Thermal cycler: Labnet - GOTAQ
Minipreps
--> 37°C/ ~10 min.
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