User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/01: Difference between revisions
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| bgcolor=#cfcfcf | Rxn 1 | | bgcolor=#cfcfcf | Rxn 1 | ||
| bgcolor=#cfcfcf | Rxn 2 | | bgcolor=#cfcfcf | Rxn 2 | ||
| rowspan="7" | [[Image:KAH050115_gel1.jpg| | | rowspan="7" | [[Image:KAH050115_gel1.jpg|200px|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA (plasmid) || 20.0 || 15.0 | | DNA (plasmid) || 20.0 || 15.0 | ||
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| dH<sub>2</sub>O || 5.0 || 10.0 | | dH<sub>2</sub>O || 5.0 || 10.0 | ||
|- | |- | ||
| || 30 μL | | || 30 μL || | ||
|} | |} | ||
--> 37°C/ ~30 min. | --> 37°C/ ~30 min. | ||
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| dH<sub>2</sub>O || 9.9 | | dH<sub>2</sub>O || 9.9 | ||
|- | |- | ||
| || 20 | | || 20 μL | ||
|} | |} | ||
--> 37°C/ 10 min.; 75°C/ 2 min.; [final] = 25 ng/μL | |||
Revision as of 13:37, 4 May 2015
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05/01/15
Assembly: KAH87 (hPcTF ORF) into MV10
--> 37°C/ ~30 min.
--> 37°C/ 10 min.; 75°C/ 2 min.; [final] = 25 ng/μL
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