User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Trasformation | * Trasformation | ||
** KAH87 is running low. | ** KAH87 is running low. | ||
** Retransform into Dh5α-turbo cells: 0.5 μL plasmid, 9.5 μL dH<sub>2</sub>O (quick protocol) | ** Retransform KAH87 and MV10 into Dh5α-turbo cells: 0.5 μL plasmid, 9.5 μL dH<sub>2</sub>O (quick protocol) | ||
** Plate on 100 μg/mL amp | ** Plate on 100 μg/mL amp | ||
Latest revision as of 00:56, 27 September 2017
Karmella's BioBrick Cloning | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||
04/29/15
MV10 Phusion PCR
Program: Phusion (block A) - edited to include 65°C - 55°C annealing gradient
Conclusion
KAH126 expression - rebuild PcTF expression plasmid for K562
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