User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/22: Difference between revisions
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'''Ryan - Receiver cloning confirmations'''<br> | '''Ryan - Receiver cloning confirmations'''<br> | ||
* Check with E/X digests | * Check with E/X digests | ||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1,2. AubR/MRV = | | rowspan="7" | <u>Expected:</u><br>1,2. AubR/MRV = ~3200, 886<br>3,4. BjaR/MRV = ~3200, 776<br>5,6. BraR/MRV = ~3200, 776<br>7,8. RpaR/MRV = ~3200, 779<br>MRV (empty) = ~3200 | ||
| rowspan="7" | <!-- [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] --> | | rowspan="7" | <!-- [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] --> | ||
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Conclusions | |||
* tba | |||
Revision as of 15:38, 23 April 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
04/22/15
Rene - minipreps & sequencing
LCR - assembly attempt #1
LCR Reactions (PCR fragments / oligo bridges)
Thermocycler: BioRad dual block
Ryan - Receiver cloning confirmations
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