User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/16: Difference between revisions
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| bgcolor=#cfcfcf | Rxn | | bgcolor=#cfcfcf | Rxn | ||
|- | |- | ||
| 100 ng Vector || | | 100 ng Vector || 0.5 | ||
|- | |- | ||
| 1:250 dsOligo || 2.0 | | 1:250 dsOligo || 2.0 | ||
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Transformation(s) | Transformation(s) | ||
* | * 10.0 μL ligation + 50 μL DH5α-turbo | ||
* Plate on 100 μg/mL amp | * Plate on 100 μg/mL amp | ||
** Split ligations in 1/2, do rapid protocl for 1 set, long protocol for other set | |||
Revision as of 17:20, 16 April 2015
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04/16/15
LCR Development
Measure conc.
Program: Phusion (block B)
Conclusion
gRNA cloning - Rene
Phosphorylate and anneal oligo pairs
Bio-Rad Thermocycler
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