User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/16

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04/16/15

  • LCR Development - PCR MV10 bacbone


LCR Development

  • Try PCR on linearized vector
Reagent Volume Expected:
1. BB 1 = size
2. BB2 = size
DNA(plasmid) 5.0 μL
10X buffer 3.0
XbaI 2.0
dH2O 20.0
  30 μL --> 37°C/ ~30 min.


Measure conc.

  • DNA purified with Sigma PCR clean-up kit
  • Eluted& back-eluted with 30 μL elution sln.
Sample OD260 260/280 ng/μL
1. MV10 XbaI 0.017 1.47 17.24




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