User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/16: Difference between revisions
From OpenWetWare
Line 93: | Line 93: | ||
| Sample || OD260 || 260/280 || ng/μL | | Sample || OD260 || 260/280 || ng/μL | ||
|- | |- | ||
| 1. 5'p-MV10 PCR || | | 1. 5'p-MV10 PCR || 0.008 || 1.28 || 7.77 | ||
|} | |} | ||
Line 143: | Line 143: | ||
Digestion/ Ligation Reactions (Vector / dsOligo Insert) | Digestion/ Ligation Reactions (Vector / dsOligo Insert) | ||
# pX330 / g051 | # pX330 / g051 | ||
# g052 | # pX330 / g052 | ||
# g053 | # pX330 / g053 | ||
# g054 | # pX330 / g054 | ||
# g055 | # pX330 / g055 | ||
# g056 | # pX330 / g056 | ||
Revision as of 17:33, 16 April 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
04/16/15
LCR Development
Measure conc.
Program: Phusion (block B)
Conclusion
gRNA cloning - Rene
Phosphorylate and anneal oligo pairs
Bio-Rad Thermocycler
|