User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/16: Difference between revisions
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|- valign="top" | |- valign="top" | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | | | bgcolor=#cfcfcf | Mix (7x) | ||
|- | |- | ||
| 100 μM Oligo 1 || 1.0 | | 100 μM Oligo 1 || 1.0 || --- | ||
|- | |- | ||
| 100 μM Oligo 2 || 1.0 | | 100 μM Oligo 2 || 1.0 || --- | ||
|- | |- | ||
| 10x T4 Lign buf (NEB) || 1.0 | | 10x T4 Lign buf (NEB) || 1.0 || 7.0 | ||
|- | |- | ||
| T4 PNK (NEB) || 0.5 | | T4 PNK (NEB) || 0.5 || 3.5 | ||
|- | |- | ||
| dH<sub>2</sub>O || 6.5 | | dH<sub>2</sub>O || 6.5 || 45.5 | ||
|- | |- | ||
| || 10.0 | | || 10.0 |
Revision as of 15:35, 16 April 2015
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04/16/15
LCR Development
Measure conc.
Program: Phusion (block B)
gRNA cloning - Rene
Phosphorylate and anneal oligo pairs
Bio-Rad Thermocycler
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