User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/08: Difference between revisions
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'''LCR - test 5'-phos primers'''<br> | '''LCR - test 5'-phos primers'''<br> | ||
# '''Gal4DB-mCh''' ( | # '''Gal4DB-mCh''' (1170 bp): Template - KAH228 plasmid, F - Gal4DB f1, R - mCh r1 | ||
# '''ATF2''' (906 bp): Template - iPSC cDNA (Brafman), F - ATF2_f1, R - ATF2_r1 | # '''ATF2''' (906 bp): Template - iPSC cDNA (Brafman), F - ATF2_f1, R - ATF2_r1 | ||
# '''MV10''' (5197 bp): Template - MV10 plasmid, F - MV10 f1, R - MV10 r1 | # '''MV10''' (5197 bp): Template - MV10 plasmid, F - MV10 f1, R - MV10 r1 | ||
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| bgcolor=#cfcfcf | Rxn3,4 | | bgcolor=#cfcfcf | Rxn3,4 | ||
| bgcolor=#cfcfcf | Rxn5,6 | | bgcolor=#cfcfcf | Rxn5,6 | ||
| rowspan="7" | Expected:<br>1,2. Gal4DB-mCh = | | rowspan="7" | Expected:<br>1,2. Gal4DB-mCh = 1170<br>3,4. ATF2 = 906<br>5,6. MV10 = 5197 | ||
| rowspan="7" | [[Image:somegel.jpg|270px|Hover name]]<br>10 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:somegel.jpg|270px|Hover name]]<br>10 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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Revision as of 16:55, 9 April 2015
 Karmella's BioBrick Cloning
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04/08/15
LCR - test 5'-phos primers
Program: GOTAQ35cyc
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