User:Karmella Haynes/Notebook/BioBrick cloning/2015/03/19: Difference between revisions
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* Build entire constructs via LCR using these as parts... | * Build entire constructs via LCR using these as parts... | ||
** '''MV10''' - XbaI-cut, contains CMV promoter, XabI, and NLS-6his-stop | ** '''MV10''' - XbaI-cut, contains CMV promoter, XabI, and NLS-6his-stop | ||
** '''Gal4DB- | ** '''Gal4DB-mCh''' - 459 bp - PCR from KAH228, 5'p oligos | ||
** '''Activation Domain''' - 159-4428 bp - PCR from cDNA, 5'p oligos | ** '''Activation Domain''' - 159-4428 bp - PCR from cDNA, 5'p oligos | ||
* Next step - order oligos | |||
** Gal4DB f / 5'p, 5'-atgaagctactgtcttctat | |||
** mCh r / 5'p, 5'-cttgtacagctcgtccatgc | |||
** LCRb_ | |||
Revision as of 12:44, 19 March 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
03/19/15
Gal4DB fusion - new strategy
Minipreps
Assemblies
Oligo annealing
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