User:Karmella Haynes/Notebook/BioBrick cloning/2015/03/19: Difference between revisions
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== | ==03/19/15== | ||
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* | * Gal4DB fusions - new strategy | ||
* | * Plasmid preps - overnight cultures | ||
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'''Gal4DB fusion - new strategy''' | |||
* Build entire constructs via LCR using these as parts... | |||
** '''MV10''' - XbaI-cut, contains CMV promoter, XabI, and NLS-6his-stop | |||
** '''Gal4DB-RFP''' - 459 bp - PCR from KAH214, 5'p oligos | |||
** '''Activation Domain''' - 159-4428 bp - PCR from cDNA, 5'p oligos | |||
Revision as of 11:49, 19 March 2015
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03/19/15
Gal4DB fusion - new strategy
Minipreps
Assemblies
Oligo annealing
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