User:Karmella Haynes/Notebook/BioBrick cloning/2015/01/27: Difference between revisions
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* Gel purification - KAH60 vector PCR, ATF2 PCR | * Gel purification - KAH60 vector PCR, ATF2 PCR | ||
* PCR - re-amplify KAH 60 vector | * PCR - re-amplify KAH 60 vector | ||
* Review LCR procedure from Cameron: http://openwetware.org/wiki/Haynes_Lab:Notebook/Engineering_PC-TFs/2014/12/22 | |||
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| bgcolor=#cfcfcf | Rxn1 | | bgcolor=#cfcfcf | Rxn1 | ||
| rowspan="7" | Expected:<br>1. KAH60 = 6637 | | rowspan="7" | Expected:<br>1. KAH60 = 6637 | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:KAH012715_gel.jpg|270px|Hover name]]<br>10 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| Template || 0 | | Template || 1.0 | ||
|- | |- | ||
| 10 uM fwd primer || 1.0 | | 10 uM fwd primer || 1.0 | ||
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| 2x GoTaq green || 12.5 | | 2x GoTaq green || 12.5 | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 9.5 | ||
|- | |- | ||
| || 25.0 | | || 25.0 |
Revision as of 10:53, 27 January 2015
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01/27/15
Gel purification
PCR
Program: GOTAQ35cyc
Assemblies
Oligo annealing
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