User:Karmella Haynes/Notebook/BioBrick cloning/2015/01/27

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01/27/15


Gel purification

  • Gel purify fragments from PCR done on 01/22/15
  • KAH60/pcVN - 6637 bp
  • ATF2 - 960 bp

PCR

  • Use purified KAH60 fragments as template for PCR
  1. KAH60 "backbone" (without VP64, includes scars) = 6637: KAH60 F1/ KAH60 R1


Reagent Rxn1 Expected:
1. KAH60 = 6637 		Hover name
10 μL/lane, 1% agarose; Ladder
Template 1.0
10 uM fwd primer 1.0
10 uM rev primer 1.0
2x GoTaq green 12.5
dH2O 9.5
  25.0

Program: GOTAQ35cyc

  • 95°C, 3 min
  • 30x[95°C, 1 min; 57°C, 1 min; 72°C, 3 min]
  • 72°C, 3 min
  • 4°C ∞



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