User:Karmella Haynes/Notebook/BioBrick cloning/2014/12/23: Difference between revisions
From OpenWetWare
Line 19: | Line 19: | ||
** PCR-amplify insert | ** PCR-amplify insert | ||
** Plan A: use LCR to ligate insert into backbone | ** Plan A: use LCR to ligate insert into backbone | ||
'''Order Primers''' (01/02/15)<br> | |||
Attempt 1: swap-in ATF activation domain; add 5' phosphates to all oligos | |||
* KAH60 "backbone" (without VP64, includes scars) = 6637 | |||
** KAH60 F1 | |||
** KAH60 R1 | |||
* ATF2 insert = 906 | |||
** ATF2_f1 | |||
** ATF2_r1 | |||
* Bridging oligos | |||
* BO_KAH60_ATF2 1 | |||
* BO_KAH60_ATF2 1 | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> |
Revision as of 12:37, 2 January 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12/23/14
Planning: Gal4 fusion cloning
Assemblies
Oligo annealing
|