09/09/13
- CMV/V0120 - minipreps, restriction digest, sequencing
- CMV/V0120
- Cultures were inoculated on 9/7/13
- Culture 1 - growth, culture 2 - growth
- Streak 1 - growth, streak 2 growth
Samples for Miniprep and Analysis
- Sample 1 = CMV/V0120 colony 1; BBa_J176027; part = 588 bp; vector = 3200 bp
- Sample 2 = CMV/V0120 colony 2; same
- Sample 3 = CMV/V0120 (control DNA); BBa_J176027; part = 588 bp; vector = 3200 bp
DNA Concentration Data
Plasmid |
OD260 |
OD260/280 |
ng/μL
|
1. Plasmid 1 |
0.609 |
1.85 |
609.4
|
2. Plasmid 2 |
0.272 |
1.88 |
272.3
|
Restriction Digest Table
- Checked plasmid minipreps with EcoRI/PstI digests
Reagent
|
Volume
|
Expected: 1. Plasmid 1 = insert size, vector size 2. Plasmid 2 = insert size, vector size
|
15 μL/lane; 1% agarose; Ladder
|
DNA(plasmid) |
3.0 μL
|
10X buffer |
1.5
|
EcoRI |
1.0
|
PstI |
1.0
|
dH2O |
8.5
|
|
15 μL --> 37°C/ 15 min.
|
DNA Sequencing Samples
- Submitted to DNASU on 'date'
- Plasmid 1 - forward primer 'name'
- Plasmid 1 - reverse primer 'name'
- Plasmid 2 - forward primer 'name'
- Plasmid 2 - reverse primer 'name'
|