User:Karmella Haynes/Notebook/BioBrick cloning/2013/09/09

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(09/09/13)
(09/09/13)
Line 36: Line 36:
| Plasmid || OD260 || OD260/280 || ng/μL
| Plasmid || OD260 || OD260/280 || ng/μL
|-
|-
-
| 1. Plasmid 1 || --- || --- || ---
+
| 1. Plasmid 1 || 0.609 || 1.85 || 609.4
|-
|-
-
| 2. Plasmid 2 || --- || --- || ---
+
| 2. Plasmid 2 || 0.272 || 1.88 || 272.3
|}
|}

Revision as of 14:28, 9 September 2013

Karmella's BioBrick Cloning Main project page
Previous entry      Next entry

09/09/13

  • CMV/V0120 - minipreps, restriction digest, sequencing



  • CMV/V0120
    • Cultures were inoculated on 9/7/13
    • Culture 1 - growth, culture 2 - growth
    • Streak 1 - growth, streak 2 growth


Samples for Miniprep and Analysis

  1. Sample 1 = CMV/V0120 colony 1; BBa_J176027; part = 588 bp; vector = 3200 bp
  2. Sample 2 = CMV/V0120 colony 2; same
  3. Sample 3 = CMV/V0120 (control DNA); BBa_J176027; part = 588 bp; vector = 3200 bp


DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 0.609 1.85 609.4
2. Plasmid 2 0.272 1.88 272.3


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on 'date'
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'



Personal tools