User:Karmella Haynes/Notebook/BioBrick cloning/2013/09/09: Difference between revisions
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| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. CMV/V0120 colony 1 = 588, 3200<br>2. CMV/V0120 colony 2 = 588, 3200<br>3. CMV/V0120 (control) = 588, 3200<br> | | rowspan="7" | <u>Expected:</u><br>1. CMV/V0120 colony 1 = 588, 3200<br>2. CMV/V0120 colony 2 = 588, 3200<br>3. CMV/V0120 (control) = 588, 3200<br> | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:Session3_JDS_digest.jpg|200px|Today's gel]]<br>[[Image:Session3_HB_digest.jpg|200px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA(plasmid) || 3.0 μL | | DNA(plasmid) || 3.0 μL | ||
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| dH<sub>2</sub>O || 8.5 | | dH<sub>2</sub>O || 8.5 | ||
|- | |- valign="top" | ||
| || 15 μL --> 37°C/ 15 min. | | || 15 μL --> 37°C/ 15 min. | ||
|} | |} | ||
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Note: Submitted along with Rene's reactions. Mine are samples 15 and 16 in the sequencing order. | Note: Submitted along with Rene's reactions. Mine are samples 15 and 16 in the sequencing order. | ||
* DNASU sequencing core power failure on 9/10/13 - data was lost | |||
* Resubmitted samples on 9/11/13 as order 8018 | |||
Revision as of 17:49, 12 September 2013
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||
09/09/13
Note: Submitted along with Rene's reactions. Mine are samples 15 and 16 in the sequencing order.
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