User:Karmella Haynes/Notebook/BioBrick cloning/2013/09/09: Difference between revisions
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'''DNA Concentration Data''' | |||
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{| {{table}} | |||
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| Plasmid || OD260 || OD260/280 || ng/μL | |||
|- | |||
| 1. Plasmid 1 || --- || --- || --- | |||
|- | |||
| 2. Plasmid 2 || --- || --- || --- | |||
|} | |||
'''Restriction Digest Table'''<br> | |||
* Checked plasmid minipreps with EcoRI/PstI digests | |||
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<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2 | |||
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| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br> | |||
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
|- | |||
| DNA(plasmid) || 3.0 μL | |||
|- | |||
| 10X buffer || 1.5 | |||
|- | |||
| EcoRI || 1.0 | |||
|- | |||
| PstI || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 8.5 | |||
|- | |||
| || 15 μL --> 37°C/ 15 min. | |||
|} | |||
'''DNA Sequencing Samples''' | |||
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples --> | |||
* Submitted to DNASU on 'date' | |||
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | |||
# Plasmid 1 - forward primer 'name' | |||
# Plasmid 1 - reverse primer 'name' | |||
# Plasmid 2 - forward primer 'name' | |||
# Plasmid 2 - reverse primer 'name' | |||
Revision as of 09:29, 9 September 2013
 Karmella's BioBrick Cloning
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