User:Karmella Haynes/Notebook/BioBrick cloning/2013/09/06: Difference between revisions
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'''DNA Concentration Data''' | |||
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| Plasmid || OD260 || OD260/280 || ng/μL | |||
|- | |||
| 1. Plasmid 1 || 0.108 || 1.841 || 108.321 | |||
|- | |||
| 2. Plasmid 2 || 0.007 || 1.75 || 6.60 | |||
|} | |||
* Streak #2 did not grow well. Culture might be empty bacteria. Use mini prep 1 for other steps. | |||
'''Restriction Digest Table'''<br> | |||
* Checked plasmid minipreps with EcoRI/PstI digests | |||
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<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2 | |||
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| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | <u>Expected:</u><br>1. CMV colony 1 = 588, 3200<br>2. CMV colony 2 = 588, 3200<br>3. CMV control = 588, 3200<br> | |||
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
|- | |||
| DNA(plasmid) || 3.0 μL | |||
|- | |||
| 10X buffer || 1.5 | |||
|- | |||
| EcoRI || 1.0 | |||
|- | |||
| PstI || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 8.5 | |||
|- | |||
| || 15 μL --> 37°C/ 15 min. | |||
|} | |||
'''DNA Sequencing Samples''' | |||
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples --> | |||
* Submitted to DNASU on 'date' | |||
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# Plasmid 1 - forward primer 'name' | |||
# Plasmid 1 - reverse primer 'name' | |||
# Plasmid 2 - forward primer 'name' | |||
# Plasmid 2 - reverse primer 'name' | |||
Revision as of 19:25, 6 September 2013
 Karmella's BioBrick Cloning
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09/06/13
Results
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