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Karmella's BioBrick Cloning
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04/18/13
- MV9 cloning - sequencing orer
MV9 sequencing
- DNASU
- DNA: 200 ng/ 10 μL
- Primers: 16 pmol (16 μM) / 10 μL
Sample |
OD260 |
260/280 |
ng/μL
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1. 1-3 |
0.054 |
1.825 |
54.37
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2. 3-5 |
0.047 |
1.73 |
47.14
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3. 3-6 |
0.131 |
1.87 |
130.79
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- Sequencing results
- MV9 1-3/ DD122B (fwd): correct sequence and orientation; reads through DD123B (rev primer) on other side
- MV9 3-5/ DD122B (fwd): correct sequence and orientation; reads through DD123B (rev primer) on other side
- MV9 3-6/ DD122B (fwd): correct sequence but backwards; reads through DD123B (rev primer) on other side
- Keep MV9 1-3 in vector stocks.
- Keep MV9 3-6 in case it might be useful
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