User:Karmella Haynes/Notebook/BioBrick cloning/2013/04/18

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(mm/dd/yy)
(04/18/13)
Line 23: Line 23:
| Sample || OD260 || 260/280 || ng/μL
| Sample || OD260 || 260/280 || ng/μL
|-
|-
-
| 1. Digested part (a/b) || --- || --- || ---
+
| 1. 1-3 || 0.054 || 1.825 || 54.37
|-
|-
-
| 2. Digested part (c/d) || --- || --- || ---
+
| 2. 3-5 || 0.047 || 1.73 || 47.14
 +
|-
 +
| 3. 3-6 || 0.131 || 1.87 || 130.79
|}
|}
 +
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Revision as of 14:11, 18 April 2013

Karmella's BioBrick Cloning Main project page
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04/18/13

  • MV9 cloning - sequencing orer



MV9 sequencing

  • DNASU
    • DNA: 200 ng/ 10 μL
    • Primers: 16 pmol (16 μM) / 10 μL


  • Measure conc.'s
Sample OD260 260/280 ng/μL
1. 1-3 0.054 1.825 54.37
2. 3-5 0.047 1.73 47.14
3. 3-6 0.131 1.87 130.79



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