User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/24: Difference between revisions
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* <font color="blue">Pre-warm agar-amp plates with lids closed</font> | * <font color="blue">Pre-warm agar-amp plates with lids closed</font> | ||
* <font color="blue">Incubate | * <font color="blue">Incubate ligations for '''30 min.'''/ room temp</font> | ||
* Add 30 μL DH5α; 5 min/ ice | * Add 30 μL DH5α; 5 min/ ice | ||
* Plate on 100 μg/mL amp; incubate at 37°C | * Plate on 100 μg/mL amp; incubate at 37°C |
Revision as of 19:54, 24 March 2013
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03/24/13
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