User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/21: Difference between revisions
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{| {{table}} cellspacing="3" <!-- Ligations table --> | {| {{table}} cellspacing="3" <!-- Ligations table --> | ||
|- bgcolor=#cfcfcf | |- bgcolor=#cfcfcf | ||
| Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/ | | Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/22/13</font> | ||
|- | |- | ||
| 1. A1 (ds oligo S/S)/76, ''0.5 μL'' + MV2(X dp)/4529, 25 ng || <font color=" | | 1. A1 (ds oligo S/S)/76, ''0.5 μL'' + MV2(X dp)/4529, 25 ng || <font color="red">MV9 lawn</font> | ||
|- | |- | ||
| 2. A1 (ds oligo S/S)/76, ''1.0 μL'' + MV2(X dp)/4529, 25 ng || <font color=" | | 2. A1 (ds oligo S/S)/76, ''1.0 μL'' + MV2(X dp)/4529, 25 ng || <font color="red">MV9 lawn</font> | ||
|- | |- | ||
| 3. A1 (ds oligo S/S)/76, ''2.0 μL'' + MV2(X dp)/4529, 25 ng || <font color=" | | 3. A1 (ds oligo S/S)/76, ''2.0 μL'' + MV2(X dp)/4529, 25 ng || <font color="red">MV9 lawn</font> | ||
|- | |- | ||
| 4. MV9(X dp)/ 25 ng || | | 4. MV9(X dp)/ 25 ng || | ||
|- | |- | ||
| 5. MV9(X no dp)/ 25 ng + Behzad's T4 ligase || <font color=" | | 5. MV9(X no dp)/ 25 ng + Behzad's T4 ligase || <font color="red">lawn</font> | ||
|- | |- | ||
| 6. MV9(X no dp)/ 25 ng || | | 6. MV9(X no dp)/ 25 ng || | ||
|} | |} | ||
{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
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* Add 30 μL DH5α; 5 min/ ice | * Add 30 μL DH5α; 5 min/ ice | ||
* Plate on 100 μg/mL amp; incubate at 37°C | * Plate on 100 μg/mL amp; incubate at 37°C | ||
3/22/13<br> | |||
* Plates have bacterial lawns. Used plates from Behzad's "LB Plane Agar" sleeve because it was marked with red tape and nobody, conveniently, reported that we ran out of LB amp. Thanks, guys. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 19:01, 24 March 2013
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
03/21/13
Assemblies
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