User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/21: Difference between revisions
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== | ==03/21/13== | ||
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* Assemblies: retry MV9 | * Assemblies: retry MV9 | ||
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{| {{table}} cellspacing="3" <!-- Ligations table --> | {| {{table}} cellspacing="3" <!-- Ligations table --> | ||
|- bgcolor=#cfcfcf | |- bgcolor=#cfcfcf | ||
| Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/ | | Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/22/13</font> | ||
|- | |- | ||
| 1. A1 (ds oligo S/S)/76, ''0.5 μL'' + MV2(X dp)/4529, 25 ng || <font color=" | | 1. A1 (ds oligo S/S)/76, ''0.5 μL'' + MV2(X dp)/4529, 25 ng || <font color="red">MV9 lawn</font> | ||
|- | |- | ||
| 2. A1 (ds oligo S/S)/76, ''1.0 μL'' + MV2(X dp)/4529, 25 ng || <font color=" | | 2. A1 (ds oligo S/S)/76, ''1.0 μL'' + MV2(X dp)/4529, 25 ng || <font color="red">MV9 lawn</font> | ||
|- | |- | ||
| 3. A1 (ds oligo S/S)/76, ''2.0 μL'' + MV2(X dp)/4529, 25 ng || <font color=" | | 3. A1 (ds oligo S/S)/76, ''2.0 μL'' + MV2(X dp)/4529, 25 ng || <font color="red">MV9 lawn</font> | ||
|- | |- | ||
| 4. MV9(X dp)/ 25 ng || | | 4. MV9(X dp)/ 25 ng || | ||
|- | |- | ||
| 5. MV9(X no dp)/ 25 ng + Behzad's T4 ligase || <font color=" | | 5. MV9(X no dp)/ 25 ng + Behzad's T4 ligase || <font color="red">lawn</font> | ||
|- | |- | ||
| 6. MV9(X no dp)/ 25 ng || | | 6. MV9(X no dp)/ 25 ng || | ||
|} | |} | ||
{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
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* Add 30 μL DH5α; 5 min/ ice | * Add 30 μL DH5α; 5 min/ ice | ||
* Plate on 100 μg/mL amp; incubate at 37°C | * Plate on 100 μg/mL amp; incubate at 37°C | ||
3/22/13<br> | |||
* Plates have bacterial lawns. Used plates from Behzad's "LB Plane Agar" sleeve because it was marked with red tape and nobody, conveniently, reported that we ran out of LB amp. Thanks, guys. | |||
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Revision as of 19:01, 24 March 2013
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03/21/13
Assemblies
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