User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/21: Difference between revisions
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* '''Oligo annealing''' | * <font color="blue">'''Oligo annealing'''</font> | ||
* See [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/BioBrick_cloning/2013/03/19 03/19/13] for details about the oligos and reaction set-up | * See [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/BioBrick_cloning/2013/03/19 03/19/13] for details about the oligos and reaction set-up | ||
** Bring ~900 mL water to a boil in a large beaker (on a hot plate). Use a thermometer to check the temperature (>100°C). | ** Bring ~900 mL water to a boil in a large beaker (on a hot plate). Use a thermometer to check the temperature (>100°C). | ||
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* | * <font color="blue">Pre-warm agar-amp plates with lids closed</font> | ||
* <font color="blue">'''30 min.'''/ room temp</font> | |||
* Add 30 μL DH5α; 5 min/ ice | * Add 30 μL DH5α; 5 min/ ice | ||
* Plate on 100 μg/mL amp; incubate at 37°C | * Plate on 100 μg/mL amp; incubate at 37°C |
Revision as of 19:49, 21 March 2013
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