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 Karmella's BioBrick Cloning
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03/06/13
- Colony PCR: for Rene's project
Colony PCR
- Streaked single colonies on gridded plates yesterday
- Used bit of streak for PCR today
| Reagent
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Volume
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Expected:
1. BB 1 = size
2. BB2 = size
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10 μL/lane; 1% agarose; Ladder
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| Bacteria from tip |
---
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| 10 μM VF2 primer |
1.0
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| 10 μM VR primer |
1.0
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| 2x GoTaq Green |
12.5
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| dH2O |
10.5
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25.0
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PCR
- 95°C, 3 min
- [95°C, 15 sec; 57°C 15 sec; 72 °C 60 sec] x35
- 72°C, 3 min
- 4°C, ∞
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Karmella's BioBrick Cloning
