User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/06: Difference between revisions
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* Streaked single colonies on gridded plates yesterday | * Streaked single colonies on gridded plates yesterday | ||
* Used bit of streak for PCR today | * Used bit of streak for PCR today | ||
* All parts are on pSB1A3-based vectors (http://partsregistry.org/Part:pSB1A3) | |||
** Use primers VF2 and VR to amplify inserts | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. | | rowspan="7" | <u>Expected:</u><br>Primers add ~250bp<br>1-3. K084007 (835 bp) = 1085<br>4-6. R0071 (53 bp) = 303<br>7. K081016 (735 bp) = 985 | ||
| rowspan="7" | [[Image:KAH030713_gel1.jpg| | | rowspan="7" | [[Image:KAH030713_gel1.jpg|300px|PCR gel]]<br>10 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| Bacteria from tip || --- | | Bacteria from tip || --- | ||
Revision as of 18:18, 7 March 2013
 Karmella's BioBrick Cloning
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03/06/13
Colony PCR
PCR
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