User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/15: Difference between revisions
From OpenWetWare
Line 37: | Line 37: | ||
9, 10. Roche - hPCD+BL01+pSB1A3<br> | 9, 10. Roche - hPCD+BL01+pSB1A3<br> | ||
11, 12. Roche - pSB1A3<br> | 11, 12. Roche - pSB1A3<br> | ||
{| {{table}} border="1" cellspacing="3" <!-- Golden Gate Rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Golden Gate Rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
Line 64: | Line 62: | ||
* 60°C, 20 min. | * 60°C, 20 min. | ||
* 80°C, 20 min. | * 80°C, 20 min. | ||
* 4°C, ∞ | |||
* Transformation | |||
** All odd samples: 50 μL '''DH5α-Turbo'''; ice 5 min.; plate on amp agar | |||
** All even samples: 50 μL '''BL21''' in 2.0 mL tubes; ice 2 min.; 42°C 90 sec.; add 800 μL SOC medium; shake @ 37°C 25 min.; pellet @ top speed 3 min.; resuspend in 100 μL amp liq. medium; plate on amp agar | |||
Revision as of 18:11, 16 January 2013
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
01/15/13
Golden Gate Assembly optimization
1, 2. Promega - hPCD+BL01+pSB1A3
Thermal cycler
|