User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/11: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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'''Minipreps'''<br>
'''Golden gate trial 1, Minipreps'''<br>
* Check with E/P digests
* Check with E/P digests


Latest revision as of 22:21, 26 September 2017

Karmella's BioBrick Cloning Main project page
Previous entry      Next entry

01/11/13

  • Golden Gate assembly trial 1: mini preps/ digests
  • Golden gate assembly trial 2: transformation


Golden gate trial 1, Minipreps

  • Check with E/P digests
Reagent Volume Expected:
1-4. hPCD-BL01/pSB1A3 = 2686, 2000 		Hover name
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL
  • --> 37°C/ ~15 min.
  • None of the digests show both expected bands; probably just vector


Golden gate assembly trial 2

  • Add 10.0 μL assembly reaction to 50.0 μL DH5α-Turbo; ice/ 5 min.; plate on amp agar



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