User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/10: Difference between revisions
From OpenWetWare
Line 22: | Line 22: | ||
'''Golden gate trial 1''' | '''Golden gate trial 1''' | ||
* Add total 10.0 μL run to 50 μL DH5α-Turbo; incubate on ice 10 min.; plate on amp agar | * Add total 10.0 μL run to 50 μL DH5α-Turbo; incubate on ice 10 min.; plate on amp agar | ||
* Colonies: 7 on assembly plate, 9 on vector-only control. Pick 4 and seed 2 mL liquid cultures. | |||
Revision as of 15:17, 11 January 2013
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
01/10/13
Violacein
Golden gate trial 1
Golden gate trial 2
PCR
Thermal cycler
|