User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/09

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| bgcolor=#cfcfcf | DNA
| bgcolor=#cfcfcf | DNA
| bgcolor=#cfcfcf | length
| bgcolor=#cfcfcf | length
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| bgcolor=#cfcfcf | Conc.
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| bgcolor=#cfcfcf | ng/μL
| bgcolor=#cfcfcf | Vol.
| bgcolor=#cfcfcf | Vol.
| bgcolor=#cfcfcf | + dH<sub>2</sub>O
| bgcolor=#cfcfcf | + dH<sub>2</sub>O

Revision as of 20:38, 9 January 2013

Karmella's BioBrick Cloning Main project page
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01/09/13

  • Golden Gate assembly: hPCD + BL01 + pSB1A3



Golden Gate Assembly

  • Use purified, measured PCR products from 1/05/13
  • Use gg2 (pSB1A3), gg3, gg4; not gg1 (V0120) since it has BsmBI sites

Dilutions

DNA length ng/μL Vol. + dH2O
gg2 pSB1A3 2000 67.7 7.7 12.3
gg3 hPCD 186 22.5 2.1 17.8
gg4 BL01 2520 55.9 11.7 8.3



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