User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/09: Difference between revisions
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* Golden Gate assembly: hPCD + BL01 + pSB1A3 | * Golden Gate assembly: hPCD + BL01 + pSB1A3 | ||
* Violacein: plates from Tabor; incubate o/n | |||
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* Golden Gate Reactions | * Golden Gate Reactions | ||
# hPCD + BL01 + pSB1A3 | |||
# pSB1A3 | |||
{| {{table}} border="1" cellspacing="3" <!-- Golden Gate Rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Golden Gate Rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
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| || 10.0 || 10.0 | | || 10.0 || 10.0 | ||
|} | |} | ||
Thermal cycler | |||
* [45°C, 2 min.; 16°C, 5 min.] x25 | |||
* 60°C, 20 min. | |||
* 80°C, 20 min. | |||
Revision as of 17:57, 10 January 2013
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01/09/13
Golden Gate Assembly
Thermal cycler
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