User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/09: Difference between revisions

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Line 24: Line 24:
| bgcolor=#cfcfcf | + dH<sub>2</sub>O
| bgcolor=#cfcfcf | + dH<sub>2</sub>O
|-
|-
| gg2 || 67.7 ||  ||  
| gg2 pSB1A3 || 67.7 ||  ||  
|-
|-
| gg3 || 22.5 ||  ||
| gg3 hPCD || 22.5 ||  ||
|-
|-
| gg4 || 55.9 ||  ||
| gg4 BL01 || 55.9 ||  ||
|}
|}


Revision as of 18:31, 9 January 2013

Karmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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01/09/13

  • Golden Gate assembly: hPCD + BL01 + pSB1A3


Golden Gate Assembly

  • Use purified, measured PCR products from 1/05/13
  • Use gg2 (pSB1A3), gg3, gg4; not gg1 (V0120) since it has BsmBI sites

Dilutions

DNA Conc. Vol. + dH2O
gg2 pSB1A3 67.7
gg3 hPCD 22.5
gg4 BL01 55.9



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