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 Karmella's BioBrick Cloning
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01/07/13
- Gibson re-transformation: grow cultures
Gibson Re-transformation Results
- Number of colonies did not improve. Only 1 colony on hPCD + BL01 + V0120 plate
- Streaks from yesterday: streak from plate 3 (hPCD + BL01 + pSB1A3 1xGibson) showed no color, whereas streak from plate 7 (hPCD + BL01 + pSB1A3 1/4x Gibson), showed RFP expression. The pSB1A3 template carries RFP.
- Grow 2x 2 mL cultures from plate 3 streak to investigate whether an insert replaced RFP.
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Karmella's BioBrick Cloning
