User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/07: Difference between revisions

Latest revision as of 22:20, 26 September 2017

Karmella's BioBrick Cloning

Gibson Re-transformation Results (1/06/13)

Number of colonies did not improve. Only 1 colony on hPCD + BL01 + V0120 plate
Streaks from yesterday: streak from plate 3 (hPCD + BL01 + pSB1A3 1xGibson) showed no color, whereas streak from plate 7 (hPCD + BL01 + pSB1A3 1/4x Gibson), showed RFP expression. The pSB1A3 template carries RFP.
Grow 2x 2 mL cultures from plate 3 streak to investigate whether an insert replaced RFP.

Gibson Re-transformation

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