User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/07
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* Gibson re-transformation: grow cultures | * Gibson re-transformation: grow cultures | ||
| + | * Re-transformation: 1x Gibson for pSB1A3 vector assemblies (3 & 4) | ||
---- | ---- | ||
| - | '''Gibson Re-transformation Results'''<br> | + | '''Gibson Re-transformation Results (1/06/13)'''<br> |
* Number of colonies did not improve. Only 1 colony on hPCD + BL01 + V0120 plate | * Number of colonies did not improve. Only 1 colony on hPCD + BL01 + V0120 plate | ||
| - | * Streaks from yesterday: | + | * Streaks from yesterday: streak from plate 3 (hPCD + BL01 + pSB1A3 1xGibson) showed no color, whereas streak from plate 7 (hPCD + BL01 + pSB1A3 1/4x Gibson), showed RFP expression. The pSB1A3 template carries RFP. |
| + | * Grow 2x 2 mL cultures from plate 3 streak to investigate whether an insert replaced RFP. | ||
| + | |||
| + | * Do Zymo minipreps | ||
| + | |||
| + | ---- | ||
| + | '''Gibson Re-transformation'''<br> | ||
| + | # hPCD + BL01 + pSB1A3 (#3), 1xGibson, '''6.0 μL''' | ||
| + | # pSB1A3 (#4), 1xGibson, '''6.0 μL''' | ||
| + | |||
| + | * Add to 50 μL cells; ice/ 10 min. | ||
| + | * Plate on 100 μg/mL amp LB agar | ||
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 Karmella's BioBrick Cloning
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01/07/13
Gibson Re-transformation Results (1/06/13)
Gibson Re-transformation
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